The Ospreys scored three tries in the last eight minutes to stun the Lions 28-38 in Johannesburg in their Challenge Cup clash on Sunday.
The Sharks continued to struggle on their overseas tour as they fell to a 19-5 defeat United Rugby Championship defeat against Ospreys at the Stoop.
Friday evening's clash between the Ospreys and Sharks at the Twickenham Stoop will mark the first time a United Rugby Championship fixture takes place in England.
Sharks Director of Rugby Neil Powell believes the team can build on a decent performance against Ospreys on Friday night.