By SABC Sport
6th June 2024
The pair were selected by Southgate as part of a 33-man training group gearing up for this summer's tournament in Germany.
Maddison was introduced as a 61st-minute substitute in Monday's 3-0 friendly win over Bosnia and Herzegovina at St James' Park, where uncapped Jones was an unused substitute.
The midfield duo will not be involved in Friday's send-off friendly against Iceland, having been informed they will not be part of the 26-man final squad submitted later that evening.
The Football Association said in a statement: "James Maddison and Curtis Jones have departed the England camp, having been told they are not part of our final Euro 2024 squad.
"We thank them for being an important part of our pre-tournament preparation."
There were 27 players involved in training on the eve of the Iceland match, with Real Madrid midfielder Jude Bellingham the only one yet to link up with England following his Champions League final win.
Anthony Gordon was able to train after missing Monday's match against Bosnia through injury, but Lewis Dunk, Harry Maguire and Luke Shaw followed individual programmes on Thursday.
Southgate has until 11pm on Friday to submit his final squad to UEFA, with the 26-man selection scheduled to be announced on Saturday morning.